Chromatography homework B- This experiment is an depth psychology of the separation of countersink pigments. The analysis is make to examine the types of pigments found in spinach plant leaves mixed with acetone. The stems of the spinach were removed. The remaining leaves were squashed to discombobulateher in an acetone-filled daub employ a pestle. later on obtaining a squirt mush, chromatography was tack together to be done. Three pieces of chromatography paper were do to a similar form to the long pointed centrifuge pipage. A capillary tube was cut at the end to absorb some of the green pigment. victimisation the tube, a dissipate of green was dabbed onto the different written document 1 cm above the tip and dipped into a solvent-filled centrifuge tube. Each paper was dipped into one of ternion solvents. The uncertain was controlled by making sure that each pointedness was the fill same green so there was an affect optical view of the results. After 15-20 mins the tube was checked. The distance from the unsanded dot to the original dot was postingd in centimeters. The diverse new colors that appeared were taken note of and compared. Limiting Factors Planning A- The purpose of this experiment was to take down the presence of amylum in two different types of leaves finished two types of methods. devil limiting factors, chlorophyll and light, were observed.
Qualitative measuring was done to measure how much starch, if any at all, was produced and how the tack reacted to light. Planning B- both plants, the coleus and plant #2, were starved of light and destarched f or 72 hours. After being starved, a stencil ! was applied to a throw from plant #2 which was then attached to a lamp. exclusively parts of the leaf except the stencil were cover with atomic number 13 foil to control the variable of light.
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